Food Lab · Microbiological analysis / principles and inquiry
Qualitative coliform guide
Link initial/final gas and follow-up evidence for nine tubes; check control validity first.
How does it work?
Gas increase alone does not confirm detection. Link follow-up evidence; control failure overrides detection evidence and invalidates the test.
Δgas = finalGas − initialGas; code ∈ {1, 0, −1, −2}
Try this investigation
- Read initial bubbles, prepare media for each inoculum volume, then observe supplied virtual results.
- Review confirmed, isolation and completed-test evidence in order for presumptive-positive tubes; record all nine tubes.
- Compare ignored initial bubbles and failed negative controls with normal records.
Interpreting your results
Should a missing completed-test result be treated as not detected?
No. Preserve null as not supplied and record pending judgment requiring more evidence. Learning review can still be completed.
Assumptions and limitations
Educational qualitative model food-edu-v1. Coliform results differ from E. coli confirmation and are not converted to CFU or MPN. This does not replace real culture or food safety assessment.